Variability of cytokine gene expression in intestinal tissue and the impact of normalization with the use of reference genes.
Identifieur interne : 000232 ( Main/Exploration ); précédent : 000231; suivant : 000233Variability of cytokine gene expression in intestinal tissue and the impact of normalization with the use of reference genes.
Auteurs : Ian Mcgowan [États-Unis] ; Laura Janocko [États-Unis] ; Shaun Burneisen [États-Unis] ; Anand Bhat [États-Unis] ; Nicola Richardson-Harman [États-Unis]Source :
- Cytokine [ 1096-0023 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN messager (génétique), Adulte, Adulte d'âge moyen, Biopsie, Chimiokines (génétique), Cytokines (génétique), Exactitude des données, Expression des gènes, Gènes essentiels, Humains, Muqueuse intestinale (immunologie), Muqueuse intestinale (métabolisme), Normes de référence, Rectum (immunologie), Rectum (métabolisme), Reproductibilité des résultats, Réaction de polymérisation en chaine en temps réel (), Sujet âgé, Sujet âgé de 80 ans ou plus, Volontaires sains.
- MESH :
- génétique : ARN messager, Chimiokines, Cytokines.
- immunologie : Muqueuse intestinale, Rectum.
- métabolisme : Muqueuse intestinale, Rectum.
- Adulte, Adulte d'âge moyen, Biopsie, Exactitude des données, Expression des gènes, Gènes essentiels, Humains, Normes de référence, Reproductibilité des résultats, Réaction de polymérisation en chaine en temps réel, Sujet âgé, Sujet âgé de 80 ans ou plus, Volontaires sains.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Biopsy, Chemokines (genetics), Cytokines (genetics), Data Accuracy, Gene Expression, Genes, Essential, Healthy Volunteers, Humans, Intestinal Mucosa (immunology), Intestinal Mucosa (metabolism), Middle Aged, RNA, Messenger (genetics), Real-Time Polymerase Chain Reaction (methods), Rectum (immunology), Rectum (metabolism), Reference Standards, Reproducibility of Results.
- MESH :
- chemical , genetics : Chemokines, Cytokines, RNA, Messenger.
- immunology : Intestinal Mucosa, Rectum.
- metabolism : Intestinal Mucosa, Rectum.
- methods : Real-Time Polymerase Chain Reaction.
- Adult, Aged, Aged, 80 and over, Biopsy, Data Accuracy, Gene Expression, Genes, Essential, Healthy Volunteers, Humans, Middle Aged, Reference Standards, Reproducibility of Results.
Abstract
To determine the intra- and inter-subject variability of mucosal cytokine gene expression in rectal biopsies from healthy volunteers and to screen cytokine and chemokine mRNA as potential biomarkers of mucosal inflammation.
DOI: 10.1016/j.cyto.2014.08.010
PubMed: 25265568
Affiliations:
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Le document en format XML
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